The oleaginous yeast Pichia manshurica isolated from Lansium domesticum fruit in Thailand and its fatty acid composition of single cell oil

Abstract. Planonth S, Chantarasiri A. 2022. The oleaginous yeast Pichia manshurica isolated from Lansium domesticum fruit in Thailand and its fatty acid composition of single cell oil. Biodiversitas 23: 801-809. Oleaginous microbes can accumulate intracellular lipids or oils (SCOs) quantities higher than 20% their biomass. They be a sustainable alternative to fossil fuels, biofuels, oleochemicals. Studies concerning efficient yeasts the natural environments remain scarce. Therefore, this study screened for surfaces longkong (Lansium domesticum) samples Thailand. Their SCOs were extracted using an ultrasonic-assisted extraction (UAE) method quantitatively analyzed. SCO-accumulating (produce amount <20 % biomass) genetically identified Candida jaroonii, Meyerozyma caribbica, Kodamaea ohmeri, sp., while >20% Hanseniaspora opuntiae. Several designated as rare microbes. P. strain Y2 was considered most effective with SCO content 43.03% (w/w). acids accumulated analyzed by gas chromatography (GC) that consisted palmitic, stearic, oleic, linoleic, palmitoleic acids. These could further applied production third-generation biodiesel, cocoa butter equivalents, related high-value